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rdfs:label "RDF description of " ;
foaf:primaryTopic .
skos:member .
skos:member .
rdf:type skos:Concept ;
"Biochemistry"@en , "Biologie moléculaire"@fr , "Molecular biology"@en , "Reproduction biology"@en , "Biologie de la reproduction"@fr , "Biochimie"@fr ;
skos:definition "Technique consistant à créer des pores dans la membrane cellulaire à l'aide d'impulsions électriques en vue de faciliter la pénétration de molécules étrangères. (Source : INRA)"@fr ;
skos:inScheme ;
skos:note "Cette technique est utilisée en transgenèse pour introduire de l'ADN dans le génome de cellules embryonnaires en culture (ex : cellules ES). (Source : INRA)"@fr , "Electroporation is a technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA. Some applications of electroporation include introduction of plasmids or foreign DNA into living cells for transfection, fusion of cells to prepare hybridomas, and insertion of proteins into cell membranes. (Source : INRA)"@en ;
skos:prefLabel "electroporation"@en , "électroporation"@fr ;
skosxl:prefLabel .
skos:member .